Transfer RNA methylating activity of yeast mitochondria

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Transfer RNA methylating activity of yeast mitochondria.

Mitochondria isolated from Saccharomyces cerevisiae and purified in Urografin or sucrose gradient contain tRNA methylating activity with specificities different from those of the cytoplasm. The main reaction product, using E.coli tRNA as methyl group acceptor, is N(2),-N(2)-dimethylguanine. The corresponding mitochondrial methylase is coded by nuclear DNA. A DNA methylating activity is also ass...

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The activities of specific enzymes for the methylation of tRNA in mammary carcinomas of the C3H mouse and the Fischer rat were compared with those in normal mammary gland. The activities of several of these en zymes were markedly increased above normal values, resulting in an alteration in the enzyme activity profile characteristic of the normal cell. Although the amount of methylase activity i...

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The DNA-dependent RNA polymerase present in the mitochondria of Saccharomyces cerevisiae has been solubilized using a 0.5 M KCI solution, and the soluble enzyme has been purified. Two forms of mitochondrial enzyme were obtained; they differ in their template specificity and metal-ion dependency. The mitochondrial RNA polymerase also differs from enzyme obtained from the nucleus with respect to ...

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Synthesis and processing of ribosomal RNA in isolated yeast mitochondria.

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Further analysis of the three-dimensional electron density map of yeast phenylalanine tRNA is presented. Attention is focused on the several types of unique hydrogen bonding that are found in the molecule and a number of sections of the electron density map are presented. These sections are compared with an electron density map of a dinucleoside phosphate. The bases in the helical stem regions ...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1974

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/1.5.707